By Leon V. Berhardt
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Additional info for Advances in Medicine and Biology, Volume 20
The sandwich magnetic immunoassay used solid-phase enzyme linked immunoassay (ELISA). The sandwich was formed by attachment of two different antibodies to different epitopes on the same target antigen (cardiac marker in this case). One antibody was attached to a solid surface used for the separation of the antigen (cardiac marker) from the blood sample. The second antibody, attached to enzyme molecules, to measure the relative concentration of glucose proportional to the cardiac troponinT marker in blood stream.
2007, 4: 127 – 129. Zhang S, Bian Z, Gu C, Zhang Y, He S, Ning Gu N, Zhang J. Preparation Colloids Surf. B. Biointerfaces. 2007, 55(2):143-148. Dengler TJ, Zimmermann R, Braun K. Müller-Bardorff M, Zehelein J, Sack FU, Schnabel PA, Kübler W, Katus HA. Elevated serum concentrations of cardiac troponin T in acute allograft rejection after human heart transplantation. J. Am. Coll. Cardiol. 1998; 32:405-412. Farina L, Iacobello C, Orlandini S, Ius A, Albertini A. The effect of the concentration ratio of avidin and biotin on a single step sandwich enzyme immunoassay.
The mixture of nanoparticle:biotin-ester: antibody solution was incubated at 45°C for 2 hours. The mixture was washed 3 times, each with 10 ml TBS, and suspended again in 10 ml TBS. In next step, anti-troponin T antibody was used for two purposes. First, fluorescence labeled with a Cy5 pigment for bioimaging similar to the method reported recently . For another experiment, maghemite nanoparticles (MNPs) were synthesized by chemical coprecipitation and coated with meso-2,3-dimercaptosuccinic acid (HOOC-CH(SH)-CH(SH)-COOH or DMSA).