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By H.D. Jakubke, J. Jeschkeit

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Because of the differences in electrophoretic mobility which arise from structural peculiarities of the individual ions, separation is also possible even when the pH 1 values are close. The mode of operation of an apparatus for continuous preparative electrophoresis is shown in Figure 4. Paper, synthetic polymers, agar gel and especially starch gel have all proved suitable carrier materials. It is conventional to distinguish between low-voltage (up to 200 V) and high-voltage electrophoresis (up to 10000 V) techniques.

The Optical Resolution of Amino Acids39 •40 In 1851 L. PASTEUR showed that synthetic amino acids and amino acids obtained from natural sources differ in their optical behaviour. The suggestion that synthetic amino acids might be an equimolar mixture of D- and L-compounds was advanced by ScHULZE and BossHARDT in 1886 as a result of microbiological experiments. In the same year A. PruTTI separated racemic DL-aspartic acid into its optical isomers by crystallisation from an aqueous solution. F. EHRLICH developed the method of optical resolution using enzymes and successfully isolated the pure D-forms of a number of important amino acids in good yield.

D-Base! 6. The Optical Resolution of Amino Acids39 •40 In 1851 L. PASTEUR showed that synthetic amino acids and amino acids obtained from natural sources differ in their optical behaviour. The suggestion that synthetic amino acids might be an equimolar mixture of D- and L-compounds was advanced by ScHULZE and BossHARDT in 1886 as a result of microbiological experiments. In the same year A. PruTTI separated racemic DL-aspartic acid into its optical isomers by crystallisation from an aqueous solution.

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